This depends on the type of plants and whether you have natural sunlight available to your garden. As a general rule, when you are in a vegetative stage of plant growth and you have no natural sunlight, run your lights 14-18 hours a day. If you have natural sunlight, it will vary because the sunlight may or may not be direct. It will take a little experimenting to find the best length of time to run your lights. If you are actively fruiting and flowering, the rule is to run your lights 12 hours a day if you have no natural light.